Cuburo environment as a diagnostic method: composition, preparation, seeding
As a nutrient medium for the cultivation of various fungi( yeast, mold), the Saburo environment can differ in composition and depending on the components used for a variety of purposes.In the practice of growing acidophilic bacteria, yeasts and pathogenic fungi, the Saburo environment is considered to be the most nutritious medium that allows using them in laboratory analyzes and studies.
What is Saburo's environment
As an artificial environment for feeding fungi, protozoa and bacteria, Saburo's environment allows you to grow and count the number and study the quality of the grown medium to control microbial contamination when using non-sterile medicines. Externally, this agent is a powder with a high degree of hygroscopicity, having a light yellow hue.
The composition of the Saburo medium can have the following:
- fish oil hydrolyzate;
- casein hydrolyzate pancreatic;
- single-substituted sodium phosphate;
- yeast extract;
Also such varieties of this type of medium can be used, su
Since the main principle of this medium is to determine the amount and speed of development of pathogenic microflora, when the number of fungi of different nature in the environment is calculated on the basis of the obtained data, as well as the degree of development of yeast and acidophilic bacteria, SaburoCan in the following cases:
- in the presence of skin lesions and, which may have a different character.With the help of the Saburo environment, it is possible to distinguish the presence on the skin of strains of bacteria and yeast fungi that cause furunculosis, skin rashes, inflammatory abscesses;
- when diagnosing the skin environment : increasing the activity of yeast fungi or acidophilic bacteria provokes frequent swelling of the tissues, hyperemia, inflammatory processes on the skin;
- for the detection of candidiasis;
- in the detection of scab, skin disease, which has a high degree of activity and is transmitted even with little contact with the infected person.
The main advantage of this procedure is its effectiveness: even with one-time inspection it becomes possible to identify many causes of skin lesions, to establish the causes of frequently recurring inflammatory processes and abscesses.
Why do such an analysis?
Saburo's medium is prescribed if complex diagnostics is necessary in detecting many skin diseases caused by active reproduction of yeast fungi, acidophilus bacteria.The appointment procedure is done by a dermatologist, and the number of procedures is also calculated by him.If it is necessary to clarify the diagnosis, two or more diagnostic procedures may be prescribed for the Saburo environment.
With repeated repetition of this research procedure, there was no adverse effect on the body.
Types of procedure
Depending on the components that are used for the analysis, the procedure may vary.The most commonly used are the following varieties:
- Saburo medium on yeast water;
- honey medium Saburo;
- peptone agar.
The listed species have a slightly altered composition, but the efficacy does not change and the reaction is used to determine the presence of an increased quantity of yeast fungi and acidophilic bacteria.
About the preparation of the environment Saburo will tell this video:
- The main indications include such conditions as various skin lesions that occur excessively often and are accompanied by aggressive manifestations such as inflammatory processes, abscesses, deep furuncles.
- Changes in both skin microflora, redness and rash also cause the appointment of this reaction for an accurate diagnosis.
- White piedra.
Contraindications for carrying out
However, with increased sensitivity of the skin to the components of the Saburo environment, this reaction is not assigned.Other methods are used to diagnose skin diseases and to choose a scheme for their treatment.
Children's age( up to a year) and pregnancy with contraindications to such studies should also be considered contraindications to the Saburo environment.
Diagram of the Saburo environment
is more safe for this study to investigate skin microflora in the absence of increased skin sensitivity, but in most cases it is safe for the human body.
For preparations for the Saburo environment, read below.
Preparing for the procedure
To ensure that the result of the analysis is not distorted, you should prepare it in advance.
There are no difficulties in preparation: on the eve it is not recommended to take spicy food, do not consume alcohol, before the procedure when taking a sample of skin, the skin surface is completely cleared. When taking any kind of medication, tell your doctor about it.
How everything passes
Special sensations in the study of the microflora of the skin do not arise, since for the analysis, scraping is taken from the skin.Particles of the skin cover all the bacteria that are detected during this reaction.
To detect violations in the composition of microflora, a scraping is taken from the surface of the skin, which is placed on Saburo's medium.Further, a reaction is performed showing the degree of occupancy of the skin by certain microorganisms( bacteria of ayidophilic origin, fungi).
Interpretation of results
For the purpose of treatment, accurate response data should be obtained, and this requires all the knowledge of the attending physician, taking into account all factors specific to the specific disease.For this reason, the process of deciphering the obtained analysis can be carried out only by the attending physician-dermatologist.
The price of Saburo's nutrient medium in microbiology is described below.
The average cost of the procedure
The price of this procedure depends on the place of its conduct, the number of repetitions of the procedure and the amount of reagents used.The cost can range from 1,280 to 1,970 rubles.
Even more useful information about the Saburo method is presented in this video: