Bacteriological sowing( seeding tank, bakpos): on flora, faeces, urine, sputum

August 12, 2017 23:00 | Methods Of Diagnosis

Bacteriological seeding( tank sowing) - a technique for studying the bacteriological component in a natural human fluid( urine, ejaculate, etc.).To determine the type of microorganisms, the material is placed in a special medium, which has a beneficial effect on their growth.If they multiply rapidly and create large colonies, the result will be positive.

A further measure is to determine the sensitivity of bacteria to antibiotics, which will allow the patient to choose a rational antibacterial therapy.Today we will tell you where it is possible to pass bacteriological culture of urine, feces, semen, sputum, etc., how to properly sow the seed in pregnancy and about such nuances.

What is bacteriological seeding

The seed tank is one of the most universal procedures by which it is possible to determine with accuracy the nature and extent of infection, to select a specific medication.At the same time, for the analysis, no special equipment is required, which significantly affects the cost, making such a

method of research available.It is also characteristic that the diagnostic technique yields accurate results.

Of particular importance is the determination of the sensitivity of pathogenic microflora to a specific type of antibiotic. Such a measure helps to accurately pick up medicines, shorten the treatment period and the negative effect of antibacterial drugs on valuable flora and immunity. The tank is seeded before antibiotics are used or after the break between courses.

It is important, in addition to revealing the sensitivity to medicines and researching the characteristics of microflora, and the quantitative evaluation.Thus, the doctor determines the approximate duration of treatment, puts a prognosis or adjusts the dosage of funds.

The following video will tell you what this bakposev is:

To whom it is assigned

Most often bacteriological sowing is assigned to women, which is associated not only with various infections of the genito-urinary organs, but also with pregnancy or conception planning.In men, this method of diagnosis also helps to identify the infection and determine the cause of infertility.

During pregnancy, the tank is seeded twice, which prevents not only the chance of infection of the woman herself, but also of the child.More often, the analysis can be performed if there is a large amount of leukocytes or protein in the blood, kidney pathology.

Control seeding is carried out after treatment.As a rule, 2-3 weeks pass from the initial stage of therapy.

About why you need to pass bacteriological culture from the cervical canal, throat, vagina and other places, read below.

Why make such an analysis

Control culture is carried out after treatment.As a rule, 2-3 weeks pass from the initial stage of therapy.

most widely Bacteriological sowings used for diagnosing infections such as:

  • candidiasis,
  • ureaplasmosis,
  • chlamydia,
  • mycoplasmosis,
  • trichomoniasis.

The procedure is necessary to determine a number of infections and inflammations.About the testimony for sowing is described in detail below.

Types of procedure

There are many species of sowing, and the disease that is to be identified plays a special role.So, sowing is carried out on:

  • mycoplasma,
  • ureaplasma,
  • dysbiosis,
  • yersiniosis,
  • flora,
  • anaerobes,
  • sterility,
  • meningococcus,
  • hemophilia,
  • gonococci,
  • yeastFungi,
  • Listeria,
  • Staphylococcus aureus,
  • Trichomonas,
  • Intestinal group,
  • Pseudomonas,
  • Hemolytic streptococci,
  • opportunistic pathogens,
  • typhoparathyphoid group.

For the seeding tank take different types of detachable:

  • cal,
  • scraping,
  • secret prostate,
  • milk breast,
  • discharge from the wound,
  • sperm,
  • synovial fluid,
  • bile,
  • smear,
  • urine,
  • sputum,
  • blood,
  • pus,
  • point.

For more information on why bacteriological seeding analysis is used in women and men, read on.

Indications for carrying out

  • selection of antibiotic;
  • diagnosis of urogenital infections;
  • preparatory procedures for conception;
  • Prostatitis;
  • vulgar impetigo;
  • itching in the intimate area;
  • diabetes mellitus;
  • black coating on the tongue;
  • anthrax;
  • resistant flora;
  • abscessed perifolliculitis,
  • treatment quality control;
  • onychomycosis;
  • pregnancy;
  • oral, exfoliative, infectious, purpurea, bullous dermatitis;
  • differential diagnostics;
  • immunodeficiency;
  • mycotic, microbial eczema;
  • gonococcal infection;
  • vulvovaginitis;
  • urethritis;
  • elevated white blood cell counts;
  • inflammatory processes.

On whether it is safe to take a smear for bacteriological culture, read on.

Preparing for a seeding tank

Tank-sow-surrender Any material is examined only before antibiotic therapy begins.The exception is re-sowing, which is necessary to control the quality of treatment.

When taking the sowing from the pharynx, it is necessary to discard the sprays, nasal and rinse ointments within a few days so as not to distort the result.On the day of the procedure, do not brush your teeth, do not eat and, if possible, refuse to drink.Before taking material from the genitals a few days before, exclude sex, do not conduct intimate hygiene with special gels, stop douching.

It is recommended to conduct standard methods of sexual hygiene before surrendering urine or ejaculate.

3-4 days before the seeding tank is better to give up alcohol, cucumbers, cabbage and other food that can affect the normal functioning of the intestines.

Contraindications and safety

No contraindications.Is the procedure safe?Completely safe.

How to pass the water tank to the urine of a child will tell this video:

How the procedure passes

The correct sampling of the separable for the study plays a special role, as this directly affects the efficiency and informative capacity of the tank.Sowing.Taking microorganisms is done only by a sterile instrument, used once, directly from the lesions.

After receiving the material necessary for the study( urine, smear mucosa, other detachable), it is placed on a nutrient medium.Further for bacteria favorable conditions are created, they are placed in a thermostat.After a short time, when they multiplied, the study of microorganisms begins.Evaluation is subject to different characteristics - density, shape, quantity, their reaction to certain types of antibiotics.

In microscopic examination, it will be necessary to differentiate the microflora.To do this, evaluate its ability to decompose some of the biochemical compositions.Sometimes microorganisms are placed on a strictly defined medium, when the task is to identify only a certain type of bacteria.For example, you can identify chlamydia if you place the material on McCoy's medium.

The antibioticogram can be carried out according to any of 3 scenarios:

  1. Diffusion method.Special strips of paper are impregnated with an antibacterial composition and placed in pathogenic microflora in a favorable environment.The degree of sensitivity is determined by the extent to which the growth of the colonies slows down.
  2. The standard disk method.The nutrient medium is in the cups, the bacteria are populated here.Special disks are impregnated with a different concentration of antibiotics and placed on top.After penetration of the medication into the medium, the level of sensitivity of microorganisms is studied.
  3. Method of serial dilutions.Sifting of microflora is carried out in test tubes with nutrient medium and biomaterial.To them antibiotics in different quantities are added.

Not always a seed tank yields accurate antibioticogram results.Some antibacterial agents that have shown high effectiveness in fighting infection in vitro are less effective at admission.This is related to the degree of bioavailability of the drug.

Next we will tell you about the decoding of bacteriological planting on the flora, etc.

Decoding of the results

Tank-seed-sbleks It takes about a week to decode the analysis - this period allows to conduct a qualitative and correct procedure that allows estimating the concentration of the pathogen in the body, its susceptibility toMedicines, etc.

Ultimately, the laboratory technician determines the degree of contamination.There are only four of them:

  1. I degree.Characterized by lean growth, which is noted exclusively in the liquid medium.
  2. II degree.Grow bacteria can already on a dense medium, only up to 10 colonies appear.
  3. III degree.There is an increase in the growth of colonies of microorganisms to 100 pieces.
  4. IV degree indicates a strong infection.In this case, the number of colonies exceeds a hundred.

The first two forms of seeding exclude the presence of the disease, and only talk about the contamination of the material.This happens when taking smears, when the patient did not pay proper attention to hygiene.On the III and IV degrees it is already possible to reveal the origin of the pathology.

Average cost of procedure

An important point is the price of bacteriological culture of urine and other materials.

The cost of a seeding tank depends on the type of microflora being determined.The price ranges from 500 to 1800 rubles.

How the tank analysis is done, the video will be described below: